白来航蛋鸡产蛋前后肝转录组功能分析

doi:10.11843/j.issn.0366-6964.2017.09.007

摘要/Abstract

摘要：

旨在从基因表达变化的角度分析蛋鸡产蛋前后肝功能变化以及这种变化对营养物质转化代谢的影响。本研究选用产蛋前1周与产蛋后1周的全同胞白来航蛋鸡作为研究对象，应用RNA-seq技术获得其产蛋前后肝转录组表达数据，经生物信息学分析，筛选出与营养物质代谢有关的重要基因。测序结果分析得出，差异表达大于两倍以上的基因有222个（P<0.05）。通过对差异表达基因进行GO富集分析和KEGG通路分析，发现所筛选的差异表达基因主要涉及脂类代谢和色氨酸代谢过程。其中，部分差异表达基因的功能尚不明确，如产蛋后肝中大量表达的环指蛋白186。通过转录组测序，得到了白来航蛋鸡产蛋前后肝差异表达基因，分析了由这些基因影响的肝中物质代谢途径变化。发现这些基因可能在蛋鸡产蛋前后通过调节肝中营养物质的代谢过程进而影响蛋鸡在产蛋后对饲料中营养物质的利用率。

Abstract:

The objective of this study was to analyze the changes of the liver metabolic function and its effects on nutrients conversion and utility based on differentially expressed gene analysis in the liver. Full-sib White Leghorn layer hens for one week before and after first egg laying were chose for liver sampling. For analyzing and screening the important genes involved in the nutrient metabolic pathway in the liver, the transcriptome data was obtained by RNA-seq technology and bioinformatics analysis, and the gene expression differences was compared before and after the first egg laying. Two hundred and twenty-two differentially expressed genes with more than 2-fold changed were obtained by the transcriptomics sequencing (P<0.05). GO enrichment analysis and KEGG pathway analysis of differentially expressed genes showed that differentially expressed genes obtained in this study mainly involved in lipid metabolism and tryptophan metabolism.In this study, there were some function-unknown differentially expressed genes were found, such as Ring finger protein 186 (RNF186). We obtained the differentially expressed genes in liver from White Leghorn hens before and after first eggs laying and analyzed the changes of metabolism pathway of nutrients in the liver. These results proved that the liver metabolism pathway of nutrients were changed by these differentially expressed genes and the changes might improve the nutrients utilization of the feed after first egg laying.

中图分类号:

S831.2

李慧锋, 张臻, 朱文进, 张同玉, 郭文婕, 蔡永强, 朱芷葳, 张利环. 白来航蛋鸡产蛋前后肝转录组功能分析[J]. 畜牧兽医学报, 2017, 48(9): 1624-1634.

LI Hui-feng, ZHANG Zhen, ZHU Wen-jin, ZHANG Tong-yu, GUO Wen-jie, CAI Yong-qiang, ZHU Zhi-wei, ZHANG Li-huan. Functional Analysis of Liver Transcriptome before and after White Leghorn Hen Begin Laying Eggs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(9): 1624-1634.

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https://www.xmsyxb.com/CN/Y2017/V48/I9/1624

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